Our 100-square-foot mouse operating room is a dedicated microsurgical area used for all mouse cardiac catheterizations and microsurgeries and contains:
- Surgical microscope
- Fiberoptic lighting
- Rodent ventilator
- Warming apparatus
- Oxygen supply
- INDUS ECG monitoring platform
Mice are transported from the housing facility directly to the surgical unit by Core personnel. The requested procedure is performed, mice are monitored for 24 hours then returned to the CSRB mouse facility.
Service and data output details
Operative placement of constriction on the transverse aorta. Includes recording mouse weight, echocardiography at study end, euthanasia at study end, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be 1-week post TAC unless requested otherwise
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.
Operative placement of a “loose” constriction on the ascending aorta of 18-22 day old mice only. Includes recording mouse weight, echocardiography at study end (8-12 weeks), euthanasia at end of study, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be two months post NAB unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.
Operative placement of constriction on ascending aorta of adult mice (preferably 18-28 gram weight). Includes recording mouse weight, 1 echo study at experiment end time, and if desired, euthanasia at study end with organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be one month post AAB unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.
Operative placement of moderate constriction on the transverse aorta combined with a small infarction caused by permanent ligation of the apical section of the left anterior descending artery. Procedure typically ventricular remodeling within a 2 week period and progression to heart failure in 4 weeks. Includes recording mouse weight, echocardiography at study end, and if desired, euthansia at study end with organ harvest and left ventricular weight measurements.
Data provided at study end: Spreadsheet with end echo parameters including regional wall differences, mouse weight before surgery, and surgical intervention (sham or HF).
Operative placement of a permanent occlusion around the left anterior descending artery of the mouse heart. Includes recording mouse weight, echocardiography at study end, and if desired, euthanasia at study end with organ harvest and left ventricular weight measurements. Additional echocardiographic studies can be requested. Study end will be one month post MI unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters including regional wall differences, mouse weight before surgery, and surgical intervention (sham or MI).
Operative placement of an occluder around the left anterior descending artery that is removed after 30-90 minutes of occlusion causing reperfusion
Operative surgical joining of two mice for parabiotic examination.
Operative surgical joining of two mice AND a subsequent surgical cardiac injury to one of the mouse pair.
Entails carotid catheter placement, fixative injection into the heart, euthanasia and organ harvest.
Price varies. For more information, please contact us.
Specialized surgery not listed above but agreed upon by surgeon and investigator.