Surgical Services

Our 100-square-foot mouse operating room is a dedicated microsurgical area used for all mouse cardiac catheterizations and microsurgeries and contains:

  • Stereotactic surgical microscope
  • Fiberoptic lighting
  • Rodent ventilator
  • Warming apparatus
  • Oxygen supply

Mice are transported from the housing facility directly to the surgical unit by Core personnel. The requested procedure is performed, mice are monitored for 24 hours then returned to the CSRB mouse facility.

Service and data output details

Transverse aortic constriction (TAC)

Operative placement of constriction on the transverse aorta. Includes recording mouse weight, echocardiography at study end, euthanasia at study end, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be 1-week post TAC unless requested otherwise

Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.

Neonatal ascending aortic banding (NAB)

Operative placement of a “loose” constriction on the ascending aorta of 18-22 day old mice only. Includes recording mouse weight, echocardiography at study end (8-12 weeks), euthanasia at end of study, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be two months post NAB unless requested otherwise.

Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.

Adult ascending aortic banding (AAB)

Operative placement of constriction on ascending aorta of adult mice (preferably 18-28 gram weight). Includes recording mouse weight, 1 echo study at experiment end time, and if desired, euthanasia at study end with organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be one month post AAB unless requested otherwise.

Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.

HF model-transverse aortic constriction with small apical infarction

Operative placement of moderate constriction on the transverse aorta combined with a small infarction caused by permanent ligation of the apical section of the left anterior descending artery. Procedure typically ventricular remodeling within a 2 week period and progression to heart failure in 4 weeks. Includes recording mouse weight, echocardiography at study end, and if desired, euthansia at study end with organ harvest and left ventricular weight measurements.

Data provided at study end: Spreadsheet with end echo parameters including regional wall differences, mouse weight before surgery, and surgical intervention (sham or HF).

Myocardial infarction (MI)

Operative placement of a permanent occlusion around the left anterior descending artery of the mouse heart. Includes recording mouse weight, echocardiography at study end, and if desired, euthanasia at study end with organ harvest and left ventricular weight measurements. Additional echocardiographic studies can be requested. Study end will be one month post MI unless requested otherwise.

Data provided at study end: Spreadsheet with end echo parameters including regional wall differences, mouse weight before surgery, and surgical intervention (sham or MI).

Myocardial ischemia/reperfusion

Operative placement of an occluder around the left anterior descending artery that is removed after 30 minutes of occlusion causing reperfusion

Parabiosis

Operative surgical joining of two mice for parabiotic examination.

Parabiosis and surgical injury (TAC or MI)

Operative surgical joining of two mice AND a subsequent surgical cardiac injury to one of the mouse pair.

Parabiotic cardiac injury

Surgical cardiac injury (TAC or MI) of one mouse of an established parabiotic pair.

Tissue fixation (in vivo heart perfusions) for histology

Entails carotid catheter placement, fixative injection into the heart, euthanasia and organ harvest.

Injections of pharmacological agents (defined by PI)

Price varies. For more information, please contact us.

Customized surgeries

Specialized surgery not listed above but agreed upon by surgeon and investigator.

 

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