Telemetry Services

Ambulatory ECG, blood pressure, activity, temperature, and blood glucose measurements can be monitored through the use of a telemetry system developed by Data Sciences International. This system eliminates the need for tethering and allows us to monitor mice for cardiac rhythm disturbances or blood pressure in the conscious mouse over long periods of time.

Monitoring is done with different types of implants and cannot be done simultaneously. Highly specialized Ponemah software is used for monitoring each of these parameters and allows us to tailor the schedule of recordings to each investigator’s needs.

With space dedicated to housing mice undergoing telemetry services, our monitoring capabilities also allow us to:

  • Collect data points continuously
  • Collect data points on a schedule (e.g. 10 sec per hr; or 5 min every 3 hr)
  • Record body temperature and activity

ECG, blood pressure, and blood glucose implant surgeries are performed under sterile conditions. After the surgery, mice are placed in a recovery area on a warmer for a period of up to one week. The scheduled recordings start 7-10 days post-surgery. Data are delivered to investigators for further analysis and we are available for consultation on all data issues.

Service and data output details

ECG

For ECG monitoring, the implant is placed sub-Q in the back and leads are placed at the left shoulder and lower left chest and tacked to the muscle using suture. For blood pressure monitoring, the skin in the neck is cut to expose the carotid artery. The carotid artery is then catheterized with a polyurethane ticking that extends from the transmitter body. This tubing contains a biocompatible gel that transduces blood pressure fluctuations back to the transmitter. The catheter is secured to the carotid artery by suture knots and a small amount of glue. The body of the transmitter is placed in a sub-Q pocket that is created by blunt dissection in the animal’s flank.

This procedure includes implantation of transmitters, post-operative single ECG recording for 24 hours and transmitter removal. Additional recording time is available. Heart rates/representative rhythm strips are provided at study end. Services do not include arrhythmia monitoring or identification of ectopy.

Data provided at study end: Summary report including surgery, survival, implant information and recording dates; spreadsheets of scheduled sampling times, heart rates and averages; printouts of representative ECG traces; and files of complete data sets upon request.

Extra Services Available with ECG Telemetry:

  • Additional recording sessions
  • Pharmacological injections during study; prices vary
  • Exercise concomitant with ECG telemetry (in addition to exercise request charges)
  • Organ harvest
  • Training in data interrogation

Blood pressure

This procedure includes implantation of transmitters and carotid artery catheterization with a polyurethane ticking. Post-operative carotid pressure recordings for a single 24 hr recording session and eventual transmitter removal are included. Blood pressure fluctuations are recorded and data is collected and categorized. Blood pressure wave forms are provided at study end.

  • Cost includes refurbishment of blood pressure catheter. Cost reflects directly the amount we are charged for refurbs and may, therefore, be increased without advanced notice if the amount charged by Data Sciences increases.

Data provided at study end: Summary report including surgery, survival, implant information and recording dates; spreadsheets of scheduled sampling times, mean pressures, systolic and diastolic pressures, pulse pressures, heart rates and averages; printouts of representative blood pressure traces; and files of complete data sets upon request.

Extra Services Available with BP Telemetry (additional charges apply):

  • Additional recording sessions
  • Pharmacological injections during study; prices vary
  • Exercise concomitant with BP telemetry (in addition to exercise request charges)
  • Organ harvest

 

Submit a requesthttps://mcpc.wustl.edu/submit-a-request/